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Product Name
Anti-Phospho-c-Jun (Ser243) Rabbit antibody
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Description
Phospho-c-Jun (Ser243) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human, Mouse, Rat
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Isotype
IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of serine 243 (P-L-S(p)-P-I) derived from Human c-Jun.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000IHC:1/50-1/100
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Validations
Western blot analysis of extracts from sorbitol-treated 293, Serum-treated C2C12 and UV-treated HepG2 cells using c-Jun (Phospho-Ser243) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (Phospho-Ser243) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot detection of c-Jun(Phospho-Ser243) in Hela cells untreated or serum-treated, using c-Jun(Phospho-Ser243) Rabbit pAb (dilution 1:500, upper) or u03b2-Actin Mouse mAb (200068-8F10, lower).Predicted band size:43kDa.Observed band size:43kDa.
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Background
Swiss-Prot Acc.P05412.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"