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Product Name
Anti-c-Jun Rabbit antibody
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Description
c-Jun Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human c-Jun
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
IP: 1/50
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Validations
Immunofluorescent analysis of Hela cells, using c-Jun Antibody .
Western blot analysis of c-Jun expression in C6 and 3T3 cell lysates using c-Jun antibody at 1/1000 dilution.Predicted band size:43KDa.Observed band size:43KDa.
Western blot analysis of c-jun expression in NIH/3T3 cell lysates.
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Background
Swiss-Prot Acc.P05412.c-jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"