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Product Name
Anti-NRF1 Rabbit antibody
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Description
NRF1 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human NRF1
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
IP: 1/50
FC: 1/50
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Validations
Immunohistochemistry assay of paraffin embedded tonsil tissue using anti-NRF1 ,showing nucleus staining.(x40)
Western blot analysis of extracts from Hela, U87MG and Jurkat cells using NRF1 rabbit pAb at 1:1000 dilution. Predicted band size: 68kDa. Observed band size: 68kDa.
Western blot analysis of extracts from K562, Ramos and Jurkat cells using NRF1 Rabbit pAb at 1:1000 dilution Predicted band size: 68kDa. Observed band size: 68kDa.
Immunocytochemistry assay of 293T using anti-NRF1 (1:250),showing nucleus staining.(x40)
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Background
Swiss-Prot Acc.Q16656.Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"