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Product Name
Anti-NOTCH3 Rabbit antibody
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Description
NOTCH3 Rabbit polyclonal antibody
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 2200 to the C-terminus of human NOTCH3 (NP_000426.2).TPVSPQERPPPYLAVPGHGEEYPAAGAHSSPPKARFLRVPSEHPYLTPSPESPEHWASPSPPSLSDWSESTPSPATATGAMATTTGALPAQPLPLSVPSSLAQAQTQLGPQPEVTPKRQVLA
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
1:500
1:2000
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Validations
Western blot - NOTCH3 Polyclonal Antibody. Western blot analysis of extracts of various cell lines, using NOTCH3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure time: 5s.
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Background
Swiss-Prot Acc.Q9UM47.This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"