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  • Anti-NGF Rabbit antibody
  • Anti-NGF Rabbit antibody

Anti-NGF Rabbit antibody

Cat.#: 167471

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-NGF Rabbit antibody
  • Documents
  • Description
    NGF Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant protein of human NGF (NP_002497.2).
  • Clonality
    Polyclonal antibody
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20°C . Avoid freeze / thaw cycles.
  • Applications

    WB: 1/500 - 1/1000

    ;IHC: 1/50 - 1/100

    ;IF: 1/50 - 1/100

  • Validations

    Immunofluorescence - NGF Polyclonal Antibody. Immunofluorescence analysis of U-251MG cells using NGF antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    Immunofluorescence - NGF Polyclonal Antibody. Immunofluorescence analysis of U-251MG cells using NGF antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    Western blot - NGF Polyclonal Antibody.Western blot analysis of extracts of various cell lines, using NGF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 60s.

    Western blot - NGF Polyclonal Antibody.Western blot analysis of extracts of various cell lines, using NGF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 60s.

  • Background
    Swiss-Prot Acc.P01138.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"