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Product Name
Anti-NGF Rabbit antibody
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Description
NGF Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human NGF (NP_002497.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20°C . Avoid freeze / thaw cycles.
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Applications
WB: 1/500 - 1/1000
;IHC: 1/50 - 1/100
;IF: 1/50 - 1/100
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Validations
Immunofluorescence - NGF Polyclonal Antibody. Immunofluorescence analysis of U-251MG cells using NGF antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot - NGF Polyclonal Antibody.Western blot analysis of extracts of various cell lines, using NGF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 60s.
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Background
Swiss-Prot Acc.P01138.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"