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Product Name
Anti-NF-kB p65 Rabbit antibody
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Description
NF-kB p65 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP, FC
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Species reactivity
Human, Mouse
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human NF-κB p65
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/5000-1/10000
IHC: 1/50-1/100
ICC/IF: 1/50-1/100
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Validations
Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder, using NF-κB p65 Antibody.
Western blot analysis of NF-kB p65 expression in MCF7,Hela,Molt-4,Raji and Jurkat cell lysates using NF-kB p65 antibody at 1/5000 dilution.Predicted band size:65KDa.Observed band size:65KDa.
Western blot analysis of NF-κB p65 expression in HeLa cell lysate.
Immunofluorescent analysis of HT-1080 cells, using NF-κB p65 Antibody .
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Background
Swiss-Prot Acc.Q04206.NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"