Anti-Phospho-NF-kB p65 (Thr254) Rabbit antibody

Cat.#: 166881

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Product Information

  • Product Name
    Anti-Phospho-NF-kB p65 (Thr254) Rabbit antibody
  • Documents
  • Description
    Phospho-NF-kB p65 (Thr254) Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Peptide sequence around phosphorylation site of threonine 254 (F-R-T(p)-P-P) derived from Human NFκB-p65.
  • Clonality
    Polyclonal
  • Formulation
    Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB:1/500-1/1000ICC:1/100-1/200IHC:1/50-1/100

  • Validations

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Thr254) Antibody  (left) or the same antibody preincubated with blocking peptide (right).

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Thr254) Antibody (left) or the same antibody preincubated with blocking peptide (right).

    Western blot analysis of extracts from 3T3 cells untreated or treated with TNF-α using NFκB-p65 (Phospho-Thr254) Antibody .

    Western blot analysis of extracts from 3T3 cells untreated or treated with TNF-α using NFκB-p65 (Phospho-Thr254) Antibody .

    Western blot detection of NFkB-p65(Phospho-Ser254) in 3T3 cells untreated or treated with Alkaline phosphatase using NFkB-p65(Phospho-Ser254) Rabbit pAb (dilution 1:500, upper) or β-Actin Mouse mAb (200068-8F10, lower).Predicted band size:65kDa.Observed band size:65KDa.

    Western blot detection of NFkB-p65(Phospho-Ser254) in 3T3 cells untreated or treated with Alkaline phosphatase using NFkB-p65(Phospho-Ser254) Rabbit pAb (dilution 1:500, upper) or β-Actin Mouse mAb (200068-8F10, lower).Predicted band size:65kDa.Observed band size:65KDa.

    Immunofluorescence staining of methanol-fixed Hela using NFκB-p65(Phospho-Thr254) Antibody .

    Immunofluorescence staining of methanol-fixed Hela using NFκB-p65(Phospho-Thr254) Antibody .

  • Background
    Swiss-Prot Acc.Q04206.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"