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Product Name
Anti-MSH6 (3E1) Mouse antibody
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Description
MSH6 (3E1) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human
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Isotype
IgG2a
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Preparation
Antigen: Purified recombinant fragment of human MSH6 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Western blot detection of MSH6 in 293 and HCT116 cell lysates using MSH6 mouse mAb (1:300-1:500 diluted).Predicted band size:160KDa.Observed band size:160KDa.
Immunocytochemistry staining of HeLa cells using MSH6 mouse mAb (dilution 1:100).
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Background
Swiss-Prot Acc.P52701.This gene encodes a protein similar to the MutS protein. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides, prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein of this gene combines with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene have been identified in individuals with hereditary nonpolyposis colon cancer (HNPCC) and endometrial cancer.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"