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Product Name
Anti-MMP2 (1E12) Mouse antibody
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Description
MMP2 (1E12) Mouse monoclonal antibody
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Tested applications
WB
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human MMP2 (AA: 242-396) expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
ELISA: 1/10000
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Validations
Western blot detection of MMP-2 in Hela,C6,MCF7,A549 and Jurkat cell lysates using MMP-2 mouse mAb (1:5000 diluted).Predicted band size:74KDa.Observed band size:72KDa.
Western blot analysis using MMP2 mAb against human MMP2 recombinant protein. (Expected MW is 42.8 kDa)
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Background
Swiss-Prot Acc.P08253.Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"