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Product Name
Anti-MLH1 Rabbit antibody
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Description
MLH1 Rabbit monoclonal antibody
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Tested applications
IHC-P, ICC/IF, FC
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Species reactivity
Human
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Isotype
IgG
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Preparation
Antigen: Recombinant protein of human MLH1
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
IHC: 1/50
ICC/IF: 1/50
FC: 1/20
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Validations
Western blot detection of MLH1 in Hela,A549,HL-60,U2OS cell lysates using MLH1 Rabbit mAb(1:1000 diluted).Predicted band size:85kDa.Observed band size:85kDa.
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Background
The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). [provided by RefSeq, Aug 2017]
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"