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  • Anti-MLH1 Rabbit antibody

Anti-MLH1 Rabbit antibody

Cat.#: 168860

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Product Information

  • Product Name
    Anti-MLH1 Rabbit antibody
  • Documents
  • Description
    MLH1 Rabbit monoclonal antibody
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    FCC2; COCA2; HNPCC; hMLH1; HNPCC2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human MLH1
  • Clonality
    Monoclonal antibody
  • Formulation
    Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
  • Storage instructions
    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    1:1000-1:5000

  • Validations

    Western blot detection of MLH1 in Rat Brain lysates using MLH1 Rabbit mAb(1:1000 diluted).Predicted band size:85kDa.Observed band size:85kDa.

    Western blot detection of MLH1 in Rat Brain lysates using MLH1 Rabbit mAb(1:1000 diluted).Predicted band size:85kDa.Observed band size:85kDa.

  • Background
    Swiss-Prot Acc.P40692.Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"