-
Product Name
Anti-IBA1/AIF1 antibody
- Documents
-
Description
Rabbit polyclonal to IBA1/AIF1
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human IBA1 / AIF1
-
Alternative names
AI607846 antibody; Aif1 antibody; AIF1 antibody; AIF-1 antibody; AIF-1 antibody; allograft inflammatory factor 1 antibody; allograft inflammatory factor 1 antibody; allograft inflammatory factor-1 splice variant Hara-1 antibody; D17H6S50E antibody; DADB-70P7.8 antibody; G1 antibody; IBA1 antibody; Iba1ionized calcium binding adapter molecule 1 antibody; interferon gamma responsive transcript antibody; ionized calcium-binding adapter molecule 1 antibody; ionized calcium-binding adapter molecule 1 antibody; IRT-1 antibody; protein G1 antibody; testis specific antibody; IRT1 antibody; IBA1 antibody; IRT1 antibody; AIF-1 antibody; IRT-1 antibody; G1 antibody; Iba1 antibody; AIF-1 antibody; AI607846 antibody; D17H6S50E antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with purified, recombinant Human IBA1 / AIF1 (rh IBA1 / AIF1; P55008-1; Met1-Pro147). IBA1 / AIF1 specific IgG was purified by Human IBA1 / AIF1 affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human IBA1 / AIF1. The detection limit for Human IBA1 / AIF1 is < 0.039 ng/well.
IP: 0.5-2 μg/mg of lysate
-
Validations
IBA1 / AIF1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
IBA1 / AIF1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
-
Background
AIF1, also known as IBA1, is an actin-binding protein. AIF1 is expressed selectively in human macrophage-like cell lines, and in a subset of CD68(+) macrophages in the interstitial and perivascular spaces of human heart allografts. It is expressed in macrophages and neutrophils. AIF1 enhances membrane ruffling and RAC activation. AIF1 enhances the actin-bundling activity of LCP1. It also enhances lymphocyte migration. AIF1 may play a role in macrophage activation and function. It binds calcium and plays a role in RAC signaling and in phagocytosis. It promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"