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Product Name
IBA1 antibody
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Description
IBA1 Rabbit Polyclonal antibody. Positive IP detected in U-937 cells. Positive WB detected in mouse spleen tissue, rat spleen tissue. Positive IHC detected in human brain tissue, human lymphoma tissue, human tonsillitis tissue. Positive IF detected in C6 cells, HepG2 cells. Observed molecular weight by Western-blot: 17 kDa
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Tested applications
ELISA, WB, IP, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AIF 1 antibody; AIF1 antibody; IBA1 antibody; IRT 1 antibody; Protein G1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IBA1 recombinant protein (Accession Number: NM_001623). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
mouse spleen tissue were subjected to SDS PAGE followed by western blot with Catalog No:111583(IBA1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:111583(IBA1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:111583(IBA1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed C6 cells using Catalog No:111583(IBA1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IP Result of anti-IBA1 (IP:Catalog No:111583, 3ug; Detection:Catalog No:111583 1:500) with U-937 cells lysate 3000ug.
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Background
AIF1, also named as G1, IBA1 or daintain/AIF-1, is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Despite a lack of detailed knowledge on the in vivo physiological functions of AIF-1, there is growing evidence that shows its aberrant expression contributes to the pathogenesis of many autoimmune diseases, including rheumatoid arthritis. (20944424). AIF1 is an actin-binding protein that enhances membrane ruffling and RAC activation. It enhances lymphocyte migration and the actin-bundling activity of LCP1. AIF1 plays a role in RAC signaling and in phagocytosis and a role in vascular inflammation. AIF1 promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. It is also as a microglial cell marker.
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References
- Li T, Feng Z, Jia S. Daintain/AIF-1 promotes breast cancer cell migration by up-regulated TNF-α via activate p38 MAPK signaling pathway. Breast cancer research and treatment. 131(3):891-8. 2012.
- Chen QR, Guan F, Yan DJ. The dynamic expression of allograft inflammatory factor-1 in hepatic tissues and splenic cells of BALB/c mice with Schistosoma japonicum infection. Tissue antigens. 79(1):33-41. 2012.
- Liao PH, Hsu YH, Yang HH, Wang MH, Chen LK. Involvement of extraneural tissues and upregulation of inducible nitric oxide synthase after experimental infection with rabies virus in BALB/c mice and LEW/SsN rats. Pathology international. 62(9):619-27. 2012.
- Elsherbiny NM, Ahmad S, Naime M. ABT-702, an adenosine kinase inhibitor, attenuates inflammation in diabetic retinopathy. Life sciences. 93(2-3):78-88. 2013.
- Zychowska M, Rojewska E, Kreiner G, Nalepa I, Przewlocka B, Mika J. Minocycline influences the anti-inflammatory interleukins and enhances the effectiveness of morphine under mice diabetic neuropathy. Journal of neuroimmunology. 262(1-2):35-45. 2013.
- Machida A, Kuwahara H, Mayra A. Intraperitoneal administration of AAV9-shRNA inhibits target gene expression in the dorsal root ganglia of neonatal mice. Molecular pain. 9:36. 2013.
- Makuch W, Mika J, Rojewska E, Zychowska M, Przewlocka B. Effects of selective and non-selective inhibitors of nitric oxide synthase on morphine- and endomorphin-1-induced analgesia in acute and neuropathic pain in rats. Neuropharmacology. 75:445-57. 2013.
- Amschl D, Neddens J, Havas D. Time course and progression of wild type α-synuclein accumulation in a transgenic mouse model. BMC neuroscience. 14:6. 2013.
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