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Anti-HP1 gamma/CBX3 (5G10) Mouse antibody

Cat.#: 168133

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Product Information

  • Product Name
    Anti-HP1 gamma/CBX3 (5G10) Mouse antibody
  • Documents
  • Description
    HP1 gamma/CBX3 (5G10) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, IP, IHC-P
  • Species reactivity
    Mouse, Human, Monkey, Hamster, Rat
  • Alternative names
    CBX 3;CBX3 ;CBX3_HUMAN;Chromobox homolog 3 (HP1 gamma homolog antibody; Drosophila);Chromobox homolog 3;Chromobox protein homolog 3 ;GAMMA; HECH;Heterochromatin like protein 1; Heterochromatin protein 1 homolog gamma;Heterochromatin protein HP1 gamma;HP1 gamma;HP1 antibody
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant human HP1-gamma protein fragments expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    ;ICC: 1/200

  • Validations

    Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-HP1-gamma mouse mAb (dilution 1:200).

    Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-HP1-gamma mouse mAb (dilution 1:200).

    Immunohistochemical analysis of paraffin-embedded Prostate Cancer using HP1-gamma mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

    Immunohistochemical analysis of paraffin-embedded Prostate Cancer using HP1-gamma mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

    Immunocytochemistry staining of HeLa cells fixed with -20u2103 Methanol and using HP1-gamma (168133,dilution 1:200) mouse mAb (green) and Histone H3.1 (Phospho-Ser10)(310045,dilution 1:200) Rabbit pAb (red). DAPI was used to stain nucleus(blue).

    Immunocytochemistry staining of HeLa cells fixed with -20u2103 Methanol and using HP1-gamma (168133,dilution 1:200) mouse mAb (green) and Histone H3.1 (Phospho-Ser10)(310045,dilution 1:200) Rabbit pAb (red). DAPI was used to stain nucleus(blue).

    Immunoprecipitation analysis of Hela cell lysates using HP1-gamma mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using HP1-gamma mouse mAb.

    Western blot detection of HP1-gamma in Hela,3T3,C6,COS7 and CHO-K1 cell lysates using HP1-gamma mouse mAb (1:1000 diluted).Predicted band size:22KDa.Observed band size:22KDa.

    Western blot detection of HP1-gamma in Hela,3T3,C6,COS7 and CHO-K1 cell lysates using HP1-gamma mouse mAb (1:1000 diluted).Predicted band size:22KDa.Observed band size:22KDa.

  • Background
    Swiss-Prot Acc.Q13185.Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"