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Product Name
Anti-Histone H3 Rabbit antibody
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Description
Histone H3 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human Histone H3
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-1/2000
IHC: 1/50-1/200
ICC/IF: 1/100-1/500
FC: 1/50
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Validations
Western blot analysis of Histone H3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) Rat brain lysate.
Immunofluorescent analysis of HeLa cells, using Histone H3 Antibody.
Immunohistochemical analysis of paraffin-embedded human colon, using Histone H3 Antibody.
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Background
Swiss-Prot Acc.P68431.Belongs to the histone H3 family. Play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"