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Product Name
Anti-Acetyl-Histone H3 (Lys14) Rabbit antibody
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Description
Acetyl-Histone H3 (Lys14) Rabbit monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP
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Species reactivity
Human, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human Histone H3 (acetyl K14)
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Clonality
Monoclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
IP: 1/50
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Validations
Western blot detection of Acetyl-Histone H3 (Lys14) in A549,HL-60 cell lysates using Acetyl-Histone H3 (Lys14) Rabbit mAb(1:1000 diluted).Observed band size:15kDa.
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Background
Swiss-Prot Acc.P68431.H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"