-
Product Name
Anti-HDAC2 Rabbit antibody
- Documents
-
Description
HDAC2 Rabbit monoclonal antibody
-
Tested applications
WB, IHC-P, ICC/IF, FC, IP
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
HD2; RPD3; YAF1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant protein of human HDAC2
-
Clonality
Monoclonal
-
Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
-
Storage instructions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze / thaw cycle.
-
Applications
1:1000
1:500
1:200
1:50
1:20
-
Validations
Western blot detection of HDAC2 in K562,C6,3T3 cell lysates using HDAC2 Rabbit mAb(1:1000 diluted).Predicted band size:55kDa.Observed band size:60kDa.
Immunocytochemistry of HDAC2(green) in Hela cells using HDAC2 Rabbit mAb at dilution 1/200, and DAPI(blue)
-
Background
Swiss-Prot Acc.Q92769.Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"