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Product Name
Anti-GSK3 beta Mouse antibody
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Description
GSK3 beta Mouse monoclonal antibody
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human, Mouse
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human GSK3B (AA: 2-159) expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody from tissue culture in PBS with 0.05% sodium azide
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Western blot analysis using GSK3B mAb against human GSK3B (AA: 2-159) recombinant protein. (Expected MW is 20.3 kDa)
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using GSK3B mouse mAb with DAB staining.
Western blot detection of Anti-GSK3B Mouse mAb in Hela,3T3,C6,Rat Brain cell lysates using Anti-GSK3B Mouse mAb(1:1000 diluted).Predicted band size:46.7KDa.Observed band size:46KDa.
Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using GSK3B mouse mAb with DAB staining.
Flow cytometric analysis of NIH/3T3 cells using GSK3B mouse mAb (green) and negative control (red).
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Background
Swiss-Prot Acc.P49841.The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"