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Product Name
Anti-Phospho-GSK3 beta (Ser9) Rabbit antibody
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Description
Phospho-GSK3 beta (Ser9) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from Human GSK3β.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000ICC:1/100-1/200IHC:1/50-1/100
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Validations
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3u03b2 (Phospho-Ser9) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3u03b2 (Phospho-Ser9) Antibody (left) or the same antibody preincubated with blocking peptide #51002 (right).
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3u03b2 (Phospho-Ser9) Antibody .
Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using GSK3u03b2 (Phospho-Ser9) Antibody .
Western blot analysis of extracts from 293 cells untreated (lane 1) or treated with serum (lane 2) using GSK3u03b2 (Phospho-Ser9) Antibody .
Western blot detection of GSK3u03b2(Phospho-Ser9) in Hela cells untreated or PDGF-treated, using GSK3u03b2(Phospho-Ser9) Rabbit pAb (dilution 1:2000, upper) or GSK3 u03b2 Mouse mAbn (200494-2E6 dilution 1:1000, lower).Predicted band size:46kDa.Observed band size:46kDa.
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Background
Swiss-Prot Acc.P49841.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"