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Product Name
Anti-Fractalkine/CX3CL1 antibody
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Description
Rabbit polyclonal to Fractalkine/CX3CL1
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Tested applications
ELISA, IHC-P
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Species reactivity
Human CX3CL1 / FKN
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Alternative names
AB030188 antibody; ABCD-3 antibody; ABCD-3 antibody; AI848747 antibody; C3Xkine antibody; NTN antibody; NTT antibody; CXC3 antibody; CXC3C antibody; SCYD1 antibody; ABCD-3 antibody; C3Xkine antibody; CX3C antibody; fractalkine antibody; neurotactin antibody; CX3C antibody; Cxc3 antibody; Scyd1 antibody; ABCD-3 antibody; AB030188 antibody; AI848747 antibody; D8Bwg0439e antibody; Cx3cl1 antibody; CX3CL1 antibody; Cxc3 antibody; CXC3 antibody; CXC3C antibody; D8Bwg0439e antibody; Fractalkine antibody; Fractalkine antibody; neurotactin antibody; NTN antibody; NTT antibody; Scyd1 antibody; SCYD1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human CX3CL1 extracellular domain (rhCX3CL1; NP_002987.1; Met 1-Arg 339). CX3CL1 specific IgG was purified by human CX3CL1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.5-1.0 μg/mL
This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human CX3CL1. The detection limit for Human CX3CL1 is 0.0195 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
Fractalkine / CX3CL1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human CX3CL1 in human brain with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to neuron.
Fractalkine / CX3CL1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human CX3CL1 in human kidney with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
Fractalkine / CX3CL1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human CX3CL1 in human appendix with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to neuron in myenteric nervous plexus. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
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Background
Fractalkine or Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a member of the CX3C chemokine family. Fractalkine / CX3CL1 is a unique chemokine that functions not only as a chemoattractant but also as an adhesion molecule and is expressed on endothelial cells activated by proinflammatory cytokines, such as interferon-gamma and tumor necrosis factor-alpha. Fractalkine/CX3CL1 is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Fractalkine / CX3CL1 is associated with dendritic cells (DC) in epidermis and lymphoid organs. The fractalkine receptor, CX3CR1, is expressed on cytotoxic effector lymphocytes, including natural killer (NK) cells and cytotoxic T lymphocytes, which contain high levels of intracellular perforin and granzyme B, and on macrophages. Soluble fractalkine causes migration of NK cells, cytotoxic T lymphocytes, and macrophages, whereas the membrane-bound form captures and enhances the subsequent migration of these cells in response to secondary stimulation with other chemokines.
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References
- Imai T, et al. (1997) Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell. 91(4): 521-30.
- Papadopoulos EJ, et al. (1999) Fractalkine, a CX3C chemokine, is expressed by dendritic cells and is up-regulated upon dendritic cell maturation. Eur J Immunol. 29 (8): 2551-9.
- Umehara H, et al. (2004) Fractalkine in vascular biology: from basic research to clinical disease". Arterioscler. Thromb Vasc Biol. 24 (1): 34-40.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"