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Product Name
Anti-eIF4G1 Rabbit antibody
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Description
eIF4G1 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: Peptide sequence around aa.1230~1234 (P-V-S-P-L) derived from Human eIF4G.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/1000ICC:1/100-1/200IHC:1/50-1/100
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Validations
Immunofluorescence staining of methanol-fixed Hela cells using eIF4G (Ab-1232) Antibody .
Western blot analysis of extracts from HepG2 and HT29 cells using eIF4G (Ab-1232) rabbit pAb (dilution 1:1000).Predicted band size:220KDa.Observed band size:220KDa.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G (Ab-1232) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from Hela and MCF7 cells using eIF4G (Ab-1232) rabbit pAb (dilution 1:1000).Predicted band size:220KDa.Observed band size:220KDa.
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Background
Swiss-Prot Acc.Q04637.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"