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Product Name
EIF4G1 antibody
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Description
EIF4G1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Positive IF detected in Hela cells, Ethacrynic acid treated HepG2 cells, HepG2 cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 250 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686A1451 antibody; eIF 4 gamma 1 antibody; eIF 4G 1 antibody; eIF 4G1 antibody; EIF4F antibody; EIF4G antibody; EIF4G1 antibody; EIF4GI antibody; p220 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF4G1 recombinant protein (Accession Number: BC007788). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110263(EIF4G1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110263(EIF4G1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using EIF4G1 antibody Catalog No:110263 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-EIF4G1 (IP:Catalog No:110263, 5ug; Detection:Catalog No:110263 1:500) with HeLa cells lysate 2000ug.
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Background
Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323].
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References
- Wu S, Wang Y, Lin L. Protease 2A induces stress granule formation during coxsackievirus B3 and enterovirus 71 infections. Virology journal. 11:192. 2014.
- Hu S, Li J, Xu F. SAMHD1 Inhibits LINE-1 Retrotransposition by Promoting Stress Granule Formation. PLoS genetics. 11(7):e1005367. 2015.
- Wehbe M, Huguenin A, Leveque N. Construction of a subgenomic CV-B3 replicon expressing emerald green fluorescent protein to assess viral replication of a cardiotropic enterovirus strain in cultured human cells. Journal of virological methods. 230:1-8. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"