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Product Name
Anti-eIF4EBP1 Rabbit antibody
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Description
eIF4EBP1 Rabbit monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
BP-1; 4EBP1; 4E-BP1; PHAS-I antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human eIF4EBP1
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/5000
IHC: 1/50
ICC/IF: 1/100
FC: 1/20
IP: 1/20
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Validations
Western blot detection of eIF4EBP1 in Jurkat,C6,CHO-K1 cell lysates using eIF4EBP1 Rabbit mAb(1:1000 diluted).Predicted band size:13kDa.Observed band size:15-20kDa.
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Background
Swiss-Prot Acc.Q13541.Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"