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Product Name
4EBP1 antibody
- Documents
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Description
4EBP1 Mouse Monoclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human pancreas tissue. Positive WB detected in K-562 cells. Observed molecular weight by Western-blot: 15-24 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse; other species not tested.
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Alternative names
4E BP1 antibody; 4EBP1 antibody; BP 1 antibody; eIF4E binding protein 1 antibody; EIF4EBP1 antibody; PHAS I antibody
- Immunogen
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of 4EBP1 recombinant protein (Accession Number: NM_004095). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:107552(4EBP1 antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human pancreas slide using Catalog No:107552(4EBP1 Antibody) at dilution of 1:50
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:107552(4EBP1 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.
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