Anti-E2F1 (4G8) Mouse antibody

Cat.#: 168448

Special Price 123.9 USD

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Product Information

  • Product Name
    Anti-E2F1 (4G8) Mouse antibody
  • Documents
  • Description
    E2F1 (4G8) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, IP
  • Species reactivity
    Human, Rat
  • Alternative names
    DmelCG6376 ; Dmel_CG6376 ; drosE2F1 ; E(Sev-CycE)3A ; E(var)3-93E ; E2-promoter binding facto ; E2F 1 ; E2F transcription factor 1 ; E2F-1 ; E2f-PA ; E2f-PB ; E2f-PC ; E2F1 ; E2f1 E2F transcription factor 1 ; E2F1_HUMAN ; Evar(3)164 ; KIAA4009 ; l(3)0717 antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    Antigen: Purified recombinant human E2F-1 protein fragments expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500

    ;ICC: 1/100

  • Validations

    Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-E2F-1 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).

    Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-E2F-1 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).

    Immunoprecipitation analysis of Hela cell lysates using E2F-1 mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using E2F-1 mouse mAb.

    Western blot detection of E2F-1 in C6,Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.

    Western blot detection of E2F-1 in C6,Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.

  • Background
    Swiss-Prot Acc.Q01094.Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"