Anti-DNA-PKcs (8D3) Mouse antibody

Cat.#: 168343

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Product Information

  • Product Name
    Anti-DNA-PKcs (8D3) Mouse antibody
  • Documents
  • Description
    DNA-PKcs (8D3) Mouse monoclonal antibody
  • Tested applications
    WB, IP, ICC/IF
  • Species reactivity
    Human
  • Alternative names
    DNA dependent protein kinase catalytic subuni;DNA PKcs;DNA-dependent protein kinase catalytic subunit;DNA-PK catalytic subunit;DNAPK;DNPK1;hyper radiosensitivity of murine scid mutation antibody; complementing 1;HYRC;HYRC1;p350;p460;PRKDC;PRKDC_HUMAN;protein kinas antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    Antigen: Purified recombinant human DNA-PKcs protein fragments expressed in E.coli
  • Clonality
    Monoclonal
  • Formulation
    Purified mouse monoclonal in PBS(pH 7.4)containing with 0.02% sodium azide 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    ;ICC: 1/200;Other 1/1000

  • Validations

    Western blot detection of DNA-PKcs in K562,Molt-4,A549 and HCT116 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.

    Western blot detection of DNA-PKcs in K562,Molt-4,A549 and HCT116 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.

    Western blot detection of DNA-PKcs in Hela,K562,HL-60 and MOLT-4 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa.

    Western blot detection of DNA-PKcs in Hela,K562,HL-60 and MOLT-4 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa.

    Immunocytochemistry stain of Hela using DNA-PKcs mouse mAb (1:200).

    Immunocytochemistry stain of Hela using DNA-PKcs mouse mAb (1:200).

    Immunoprecipitation analysis of Hela cell lysates using DNA-PKcs mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using DNA-PKcs mouse mAb.

  • Background
    Swiss-Prot Acc.P78527.The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"