Cart 2

Anti-DNA-PKcs (6D1) Mouse antibody

Cat.#: 168261

Size:

Special Price 123.9 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Anti-DNA-PKcs (6D1) Mouse antibody
  • Documents
  • Description
    DNA-PKcs (6D1) Mouse monoclonal antibody
  • Tested applications
    WB, IP, ICC/IF, IHC-P
  • Species reactivity
    Human
  • Alternative names
    DNA dependent protein kinase catalytic subuni;DNA PKcs;DNA-dependent protein kinase catalytic subunit;DNA-PK catalytic subunit;DNAPK;DNPK1;hyper radiosensitivity of murine scid mutation antibody; complementing 1;HYRC;HYRC1;p350;p460;PRKDC;PRKDC_HUMAN;protein kinas antibody
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant human DNA-PKcs protein fragments expressed in E.coli
  • Clonality
    Monoclonal
  • Formulation
    Purified mouse monoclonal antibody in PBS(pH 7.4)containing with 0.02% sodium azide 0.1%BSA 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    ;ICC: 1/100;Other 1/1000

  • Validations

    Immunoprecipitation analysis of Hela cell lysate using DNA-PKcs mouse mAb.

    Immunoprecipitation analysis of Hela cell lysate using DNA-PKcs mouse mAb.

    Immunohistochemical analysis of paraffin-embedded Breast cancer using DNA-PKcs mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

    Immunohistochemical analysis of paraffin-embedded Breast cancer using DNA-PKcs mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

    Western blot detection of DNA-PKcs in Hela and K562 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa.

    Western blot detection of DNA-PKcs in Hela and K562 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa,Observed band size:450KDa.

    Western blot detection of DNA-PKcs in Hela,Molt-4,A549 and HCT116 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.

    Western blot detection of DNA-PKcs in Hela,Molt-4,A549 and HCT116 cell lysates using DNA-PKcs mouse mAb (1:1000 diluted).Predicted band size:450KDa.Observed band size:450KDa.

    Immunocytochemistry stain of Hela using DNA-PKcs mouse mAb (1:100).

    Immunocytochemistry stain of Hela using DNA-PKcs mouse mAb (1:100).

  • Background
    Swiss-Prot Acc.P78527.Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage.Involved in DNA nonhomologous end joining(NHEJ)required for double-strand break(DSB)repair and V(D)J recombination.Must be bound to DNA to express its catalytic properties.Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis(DCLRE1C).The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step.Required to protect and align broken ends of DNA.May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage.Found at the ends of chromosomes,suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion.Also involved in modulation of transcription.Recognizes the substrate consensus sequence[ST]-Q.Phosphorylates'Ser-139' of histone variant H2AX/H2AFX,thereby regulating DNA damage response mechanism.Phosphorylates DCLRE1C,c-Abl/ABL1,histone H1,HSPCA,c-jun/JUN,p53/TP53, PARP1, POU2F1,DHX9,SRF,XRCC1,XRCC1,XRCC4,XRCC5,XRCC6,WRN,MYC and RFA2.Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA.Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"