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Product Name
Anti-Cyclin B1 Mouse antibody
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Description
Cyclin B1 Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human, Rat
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human CCNB1 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Western blot analysis using CCNB1 mouse mAb against Hela (1) and PC-12 (2) cell lysate.
Immunofluorescence analysis of Hela cells using CCNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of Hela cells using CCNB1 mouse mAb (green) and negative control (purple).
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Background
Swiss-Prot Acc.P14635.The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. (provided by RefSeq) It has higher expression in tumor tissues .
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"