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Product Name
Anti-Cyclin B1 Rabbit antibody
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Description
Cyclin B1 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human Cyclin B1
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
FC: 1/100
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Validations
Western blot detection of Cyclin B1 in 293T,Jurkat,Hela cell lysates using Cyclin B1 Rabbit pAb(1:1000 diluted).Predicted band size:55kDa.Observed band size:55kDa.
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Background
Swiss-Prot Acc.P14635.Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"