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Product Name
Anti-COTL1 (4E11) Mouse antibody
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Description
COTL1 (4E11) Mouse monoclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human COTL1 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ELISA: 1/10000
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Validations
Immunohistochemical analysis of paraffin-embedded breast cancer tissues using COTL1 mouse mAb with DAB staining.
Western blot detection of COTL1 in MCF7 and C6 cell lysates using COTL1 mouse mAb (1:1000 diluted).Predicted band size:16KDa.Observed band size:16KDa.
Western blot analysis using COTL1 mAb against HEK293 (1) and COTL1 (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using COTL1 mouse mAb with DAB staining.
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Background
Swiss-Prot Acc.Q14019.This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes.
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