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Product Name
COTL1 antibody
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Description
COTL1 Mouse Monoclonal antibody. Positive WB detected in fetal human brain tissue, human brain tissue. Positive IHC detected in human kidney tissue, human lung cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 16 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human,Mouse; other species not tested.
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Alternative names
CLP antibody; Coactosin like protein antibody; COTL1 antibody; FLJ43657 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of COTL1 recombinant protein (Accession Number: NM_021149). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
fetal human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107170(COTL1 Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:107170(COTL1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:107170(COTL1 Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells using Catalog No:107170(COTL1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"