Anti-CD44 (8E2) Mouse antibody
CD44 (8E2) Mouse monoclonal antibody
WB, IHC-P, ICC/IF, FC
Antigen: Purified recombinant fragment of human CD44 (628-699) expressed in E. Coli.
Ascitic fluid containing 0.03% sodium azide.
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ICC: 1/200 - 1/1000
FC: 1/200 - 1/400
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using CD44 mouse mAb (dilution 1:100).
Western blot detection of CD44 in COS7,HUVEC and Hela cell lysates using CD44 mouse mAb (dilution 1:1000).Predicted band size:82 Kda.Observed band size:82KDa.
Swiss-Prot Acc.P16070.CD44, also known as IN, LHRMIC4, CDW44, HCELL. It is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
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