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Anti-Anti-CD44 Mouse antibody

Cat.#: 169110

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Special Price 461.6 USD

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Product Information

  • Product Name
    Anti-Anti-CD44 Mouse antibody
  • Documents
  • Description
    Anti-CD44 Mouse monoclonal antibody
  • Tested applications
    IHC-P, WB, ICC/IF, FC
  • Species reactivity
    Human
  • Isotype
    Mouse IgG
  • Preparation
    Antigen: This CD44 monoclonal antibody is generated from mouse immunized with CD44 recombinant protein.
  • Clonality
    Monoclonal
  • Formulation
    Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Applications

    WB::1:2000ICC::1:10~50

  • Validations

    169110 staining CD44 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

    169110 staining CD44 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

    CD44 antibody (Cat. #169110) confocal immunofluorescent analysis with hela cell. 0.01 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits red fluorescence. DAPI was used to stain the cell nuclear (blue).

    CD44 antibody (Cat. #169110) confocal immunofluorescent analysis with hela cell. 0.01 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits red fluorescence. DAPI was used to stain the cell nuclear (blue).

    169110 staining CD44 in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

    169110 staining CD44 in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

    Western blot detection of CD44 in A549 and Hela cells using CD44 Mouse mAb(dilution 1:1000).Predicted band size:81kDa.Observed band size:81kDa.

    Western blot detection of CD44 in A549 and Hela cells using CD44 Mouse mAb(dilution 1:1000).Predicted band size:81kDa.Observed band size:81kDa.

  • Background
    Swiss-Prot Acc.P16070.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"