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Anti-Bcl10 (6F7) Mouse antibody

Cat.#: 168417

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-Bcl10 (6F7) Mouse antibody
  • Documents
  • Description
    Bcl10 (6F7) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, FC
  • Species reactivity
    Human, Mouse
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human BCL-10 expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    IHC: 1/200 - 1/1000

    ICC: 1/200 - 1/1000

    FC: 1/200 - 1/400

    ELISA: 1/10000

  • Validations

    Western blot detection of Bcl10 in Raj,Jurkat and MCF7 cell lysates using Bcl10 mouse mAb (1:1000 diluted).Predicted band size:26KDa.Observed band size:26KDa.

    Western blot detection of Bcl10 in Raj,Jurkat and MCF7 cell lysates using Bcl10 mouse mAb (1:1000 diluted).Predicted band size:26KDa.Observed band size:26KDa.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A) and liver carcinoma (B), showing cytoplasmic localization using BCL10 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A) and liver carcinoma (B), showing cytoplasmic localization using BCL10 mouse mAb with DAB staining.

    Confocal immunofluorescence analysis of Hela cells using BCL10 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

    Confocal immunofluorescence analysis of Hela cells using BCL10 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

    Flow cytometric analysis of Hela cells using BCL10 mouse mAb (green) and negative control (purple).

    Flow cytometric analysis of Hela cells using BCL10 mouse mAb (green) and negative control (purple).

  • Background
    Swiss-Prot Acc.O95999.Bcl-10 (B-cell CLL/lymphoma 10), also known as CLAP, Me10, CIPER, c-E10, CARMEN. Entrez Protein NP_003912. It is a protein containing a caspase recruitment domain (CARD). It plays an important role in apoptosis and activating NF-kappaB. The research suggested that it interacted with other CARD domain containing proteins including CARD9, 10, 11 and 14, which were thought to function as upstream regulators in NF-kappaB signaling. Bcl-10 is found to form a complex with MALT1 which encoded by another gene known to be translocated in MALT lymphoma. MALT1 and Bcl-10 are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"