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Product Name
Anti-Bak Rabbit antibody
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Description
Bak Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP, FC
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human Bak
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
1:500~1:2000
1:50~1:200
1:50~1:200
1:50
1:50
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Validations
Immunofluorescent analysis of Hela cells, using Bak Antibody .
Western blot analysis of extracts of HeLa cell lysate, using BAK1 antibody.
Immunohistochemical analysis of paraffin-embedded mouse liver, using Bak Antibody.
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Background
Swiss-Prot Acc.Q16611.Bak is a proapoptotic member of the Bcl-2 family. This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway. Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release. The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"