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Product Name
Anti-Bag1 Rabbit antibody
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Description
Bag1 Rabbit monoclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
HAP; BAG-1; RAP46 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human Bag1
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
IHC: 1/20
ICC/IF: 1/50
IP: 1/20
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Validations
Western blot detection of Bag1 in Hela,MCF7,U251MG,K562 cell lysates using Bag1 Rabbit mAb(1:500 diluted).Predicted band size:39kDa.Observed band size:52,46,33kDa.
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Background
Swiss-Prot Acc.Q99933.Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:27474739, PubMed:9873016, PubMed:24318877). Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity (PubMed:12724406). Markedly increases the anti-cell death function of BCL2 induced by various stimuli (PubMed:9305631).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"