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Product Name
BAG1 antibody
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Description
BAG1 Rabbit Polyclonal antibody. Positive IF detected in Hela cells, MCF-7 cells. Positive IHC detected in human testis tissue, human ovary tissue. Positive WB detected in HeLa cells, HEK-293 cells, HL-60 cells, HT-1080 cells, Jurkat cells, MCF7 cells, RAW264.7 cells, UV treated Hela cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 50-55 kDa,30-33 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BAG 1 antibody; BAG1 antibody; BAG1L speicfic antibody; BAG1M antibody; BAG1S antibody; HAP antibody; RAP46 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BAG1 recombinant protein (Accession Number: BC014774). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108406(BAG1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using Catalog No:108406(BAG1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:108406(BAG1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using BAG1 antibody Catalog No:108406 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-BAG1 (IP:Catalog No:108406, 3ug; Detection:Catalog No:108406 1:500) with HeLa cells lysate 3440ug.
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Background
BAG1 have been identified that modulate gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody can recognize all the isoforms of BAG1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"