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Anti-AMPK beta 1 (1A7) Mouse antibody

Cat.#: 168476

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Product Information

  • Product Name
    Anti-AMPK beta 1 (1A7) Mouse antibody
  • Documents
  • Description
    AMPK beta 1 (1A7) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, IP, IHC-P
  • Species reactivity
    Human, Mouse, Rat, Monkey
  • Alternative names
    1300015D22Rik;5 AMP activated protein kinase subunit beta 1;5''-AMP-activated protein kinase subunit beta-1;AAKB1_HUMAN;AMP-ACTIVATED PROTEIN KINASE antibody; NONCATALYTIC antibody; BETA-1; AMP-activated antibody; noncatalytic antibody; beta-1;AMPK;AMPK beta 1 chain;AMPK subunit beta-1;AMPK antibody
  • Isotype
    Mouse IgG2a
  • Preparation
    Antigen: Purified recombinant human AMPK beta 1 protein fragments expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    ;ICC: 1/100

  • Validations

    Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPK beta 1 mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

    Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPK beta 1 mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

    Western blot detection of AMPK beta 1 in 3T3,Hela,PC-12,COS7 and MDA-MB-468 cell lysates using AMPK beta 1 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.Exposure time:5min.

    Western blot detection of AMPK beta 1 in 3T3,Hela,PC-12,COS7 and MDA-MB-468 cell lysates using AMPK beta 1 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.Exposure time:5min.

    Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using AMPK beta 1 mouse mAb (dilution 1:100).

    Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using AMPK beta 1 mouse mAb (dilution 1:100).

    Immunoprecipitation analysis of Hela cell lysates using AMPK beta 1 mouse mAb.

    Immunoprecipitation analysis of Hela cell lysates using AMPK beta 1 mouse mAb.

  • Background
    Swiss-Prot Acc.Q9Y478.AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2; β1, 2; γ1, 2, 3).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"