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Product Name
Anti-Alkaline Phosphatase Rabbit antibody
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Description
Alkaline Phosphatase Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human Alkaline Phosphatase
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/5000-1/10000
IHC: 1/50-1/200
ICC/IF: 1/50-1/100
IP: 1/50
FC: 1/50
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Validations
Western blot analysis of extracts from SKOV-3, Jurkat and MCF7 cells using Alkaline Phosphatase Rabbit pAb at 1:1000 dilution. Predicted band size: 57kDa. Observed band size: 70kDa.
Immunohistochemical analysis of paraffin-embedded human liver, using Alkaline Phosphatase Antibody.
Western blot analysis of Alkaline Phosphatase expression in (1)HepG2 cell lysate; (2)JAR cell lysate.
Immunofluorescent analysis of Hela cells, using Alkaline Phosphatase Antibody .
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Background
Swiss-Prot Acc.P05186.There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"