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Product Name
ALDH1A1 antibody
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Description
ALDH1A1 Mouse Monoclonal antibody. Positive IF detected in A431 cells, HeLa cells. Positive IHC detected in human brain tissue. Positive WB detected in MCF7 cell, rat liver tissue. Observed molecular weight by Western-blot: 52 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
ALDC antibody; ALDH E1 antibody; ALDH1 antibody; ALDH11 antibody; ALDH1A1 antibody; ALHDII antibody; PUMB1 antibody; RALDH 1 antibody; RALDH1 antibody; Retinal dehydrogenase 1 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of ALDH1A1 recombinant protein (Accession Number: NM_000689). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:50-1:500
IF: 1:10-1:100
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:107570(ALDH1A1 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:107570(ALDH1A1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Sodium Citrate buffer (pH6).
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:107570(ALDH1A1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Sodium Citrate buffer (pH6).
Immunofluorescent analysis of A431 cells using Catalog No:107570(ALDH1A1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG
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Background
ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1A1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"