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Product Name
ADRP/Perilipin 2 antibody
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Description
ADRP/Perilipin 2 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human colon cancer tissue, human liver cancer tissue, human renal cell carcinoma tissue. Positive IF detected in HepG2 cells, 3T3-L1 cells. Positive WB detected in Transfected HEK-293 cells. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ADFP antibody; Adipophilin antibody; ADRP antibody; Perilipin 2 antibody; PLIN2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ADRP/Perilipin 2 recombinant protein (Accession Number: NM_001122). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human liver using Catalog No:107895(ADRP antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:107895(ADRP Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107895(ADRP Antibody) at dilution of 1:2000
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Background
ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.
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