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Product Name
14-3-3 epsilon antibody
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Description
14-3-3 epsilon Rabbit Polyclonal antibody. Positive IP detected in A375 cells. Positive WB detected in A375 cells, HeLa cells, human heart tissue. Positive IHC detected in human gliomas tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB, IHC, IF, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
14 3 3 protein epsilon antibody; 14 3 3E antibody; 14-3-3 antibody; 14-3-3 epsilon antibody; FLJ45465 antibody; KCIP 1 antibody; MDCR antibody; MDS antibody; YWHAE antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of 14-3-3 epsilon recombinant protein (Accession Number: NM_006761). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:107659(14-3-3E antibody) at dilution of 1:500
IP Result of anti-14-3-3E (IP:Catalog No:107659, 3ug; Detection:Catalog No:107659 1:1000) with A375 cells lysate 6000ug.
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:107659(14-3-3E antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using YWHAE antibody Catalog No:107659 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HepG2 cells were stained with 0.2ug 14-3-3E antibody (Catalog No:107659, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
14-3-3 epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against full-length 14-3-3 epsilon.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"