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Product Name
ZP3 antibody
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Description
ZP3 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue, human prostate cancer tissue. Positive IP detected in HeLa cells. Positive WB detected in multi-cells. Observed molecular weight by Western-blot: 46 kDa, 60 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
Sperm receptor antibody; Zona pellucida glycoprotein 3 antibody; Zona pellucida protein C antibody; Zp 3 antibody; ZP3 antibody; ZP3A antibody; ZP3A/ZP3B antibody; ZP3B antibody; ZPC antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ZP3 recombinant protein (Accession Number: NM_007155). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
WB result of Catalog No:117262(ZP3) from Dr. Soory. (Target band 41-47kd,56kd(Glycosylated form, PMID:19700799),83kd(PMID: 1577197).
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:117262(ZP3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:117262(ZP3 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-ZP3 (IP:Catalog No:117262, 4ug; Detection:Catalog No:117262 1:500) with HeLa cells lysate 1600ug.
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Background
Zona pellucida (ZP) is an extracellular matrix that surrounds the oocyte and early embryo, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. ZP3 (zona pellucida glycoprotein 3, also known as zona pellucida sperm-binding protein 3) is essential for sperm binding and zona matrix formation.
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