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Product Name
ZNRF3 Polyclonal Antibody
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Description
Polyclonal antibody to ZNRF3
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
ZNRF3 antibody; BK747E2.3 antibody; RNF203 antibody; zinc and ring finger 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ZNRF3 (NP_115549.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - ZNRF3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ZNRF3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunohistochemistry - ZNRF3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using ZNRF3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNRF3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using ZNRF3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNRF3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver using ZNRF3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNRF3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse spleen using ZNRF3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ZNRF3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using ZNRF3 antibody at dilution of 1:100 (40x lens).
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Background
E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone. Along with RSPO2 and RNF43, constitutes a master switch that governs limb specification (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"