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ZEB1 antibody

Cat.#: 117037

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Product Information

  • Product Name
    ZEB1 antibody
  • Documents
  • Description
    ZEB1 Rabbit Polyclonal antibody. Positive IP detected in MCF-7 cells. Positive WB detected in MCF7 cells, HeLa cells, PC-3 cells. Observed molecular weight by Western-blot: 190-210 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    AREB6 antibody; BZP antibody; DELTA EF1 antibody; delta EF1 antibody; Negative regulator of IL2 antibody; NIL 2 A antibody; NIL 2 A zinc finger protein antibody; NIL 2A antibody; NIL2A antibody; TCF 8 antibody; TCF8 antibody; Transcription factor 8 antibody; ZEB antibody; ZEB1 antibody; ZFHEP antibody; ZFHX1A antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ZEB1 recombinant protein (Accession Number: NM_001174096). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

  • Validations

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:117037(ZEB1 antibody) at dilution of 1:500

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:117037(ZEB1 antibody) at dilution of 1:500

    IP Result of anti-ZEB1 (IP:Catalog No:117037, 4ug; Detection:Catalog No:117037 1:500) with MCF-7 cells lysate 4000ug.

    IP Result of anti-ZEB1 (IP:Catalog No:117037, 4ug; Detection:Catalog No:117037 1:500) with MCF-7 cells lysate 4000ug.

  • Background
    ZEB1(zinc-finger-enhancer binding protein 1), which is a members of the Zincfinger-Homeobox (ZFH)-family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene . Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post phosphorylation of protein is about 190-210 kDa. This is a rabbit polyclonal antibody raised against part of the full length of ZEB1 of human.
  • References
    • Zhuang J, Lu Q, Shen B. TGFβ1 secreted by cancer-associated fibroblasts induces epithelial-mesenchymal transition of bladder cancer cells through lncRNA-ZEB2NAT. Scientific reports. 5:11924. 2015.
    • OuYang HY, Xu J, Luo J. MEP1A contributes to tumor progression and predicts poor clinical outcome in human hepatocellular carcinoma. Hepatology (Baltimore, Md.). 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"