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Product Name
ZDHHC13 antibody
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Description
ZDHHC13 Rabbit Polyclonal antibody. Positive IF detected in HEK-293 cells. Positive IHC detected in human stomach tissue, human testis tissue. Positive WB detected in mouse kidney tissue, HEK-293 cells, mouse skin tissue, mouse testis tissue, Neuro-2a cells. Observed molecular weight by Western-blot: 45-50 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DHHC 13 antibody; FLJ10852 antibody; FLJ10941 antibody; HIP14 related protein antibody; HIP14L antibody; HIP3RP antibody; Palmitoyltransferase ZDHHC13 antibody; ZDHHC13 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ZDHHC13 recombinant protein (Accession Number: NM_019028). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:116938(ZDHHC13 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human stomach tissue slide using Catalog No:116938(ZDHHC13 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:116938(ZDHHC13 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:116938(ZDHHC13 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Palmitoyltransferase ZDHHC13 also named as HIP14L or DHHC13 is a 622 amino acid protein, which contains one DHHC-type zinc finger and six ANK repeats. ZDHHC13 localizes in the Golgi apparatus membrane and belongs to the DHHC palmitoyltransferase family. ZDHHC13 is a palmtoyltransferase for HD and GAD2, meanwhile it mediates Mg2+ transportation. ZDHHC13 exists as three alternative splicing isoforms. The molecular weight of isoform 2 is 51 kDa, but we often detected a 45-50kDa protein by western blot.
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