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ZCCHC11 antibody

Cat.#: 116932

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Product Information

  • Product Name
    ZCCHC11 antibody
  • Documents
  • Description
    ZCCHC11 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IP detected in MCF-7 cells. Positive WB detected in MCF7 cells, HEK-293T cells, HeLa cells, human brain tissue. Observed molecular weight by Western-blot: 185 kDa
  • Tested applications
    ELISA, WB, IF, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    KIAA0191 antibody; PAPD3 antibody; Terminal uridylyltransferase 4 antibody; TUT4 antibody; TUTase 4 antibody; ZCCHC11 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_015269). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:500-1:5000

    IF: 1:20-1:200

  • Validations

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:116932(ZCCHC11 antibody) at dilution of 1:500

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:116932(ZCCHC11 antibody) at dilution of 1:500

    IP Result of anti-ZCCHC11 (IP:Catalog No:116932, 5ug; Detection:Catalog No:116932 1:1000) with MCF-7 cells lysate 2000ug.

    IP Result of anti-ZCCHC11 (IP:Catalog No:116932, 5ug; Detection:Catalog No:116932 1:1000) with MCF-7 cells lysate 2000ug.

    Immunofluorescent analysis of HeLa cells using Catalog No:116932(ZCCHC11 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG

    Immunofluorescent analysis of HeLa cells using Catalog No:116932(ZCCHC11 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG

  • Background
    ZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can’t bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.This antibody is specifical to the 185 kd ZCCHCC11 protein.
  • References
    • Piskounova E, Polytarchou C, Thornton JE. Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms. Cell. 147(5):1066-79. 2011.
    • Chang HM, Triboulet R, Thornton JE, Gregory RI. A role for the Perlman syndrome exonuclease Dis3l2 in the Lin28-let-7 pathway. Nature. 497(7448):244-8. 2013.
    • Thornton JE, Du P, Jing L. Selective microRNA uridylation by Zcchc6 (TUT7) and Zcchc11 (TUT4). Nucleic acids research. 42(18):11777-91. 2014.
    • Choudhury NR, Nowak JS, Zuo J, Rappsilber J, Spoel SH, Michlewski G. Trim25 Is an RNA-Specific Activator of Lin28a/TuT4-Mediated Uridylation. Cell reports. 9(4):1265-72. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"