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Product Name
ZAP70 antibody
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Description
ZAP70 Rabbit Polyclonal antibody. Positive FC detected in Raji cells. Positive IF detected in RAW 264.7 cells. Positive IHC detected in human lymphoma tissue. Positive WB detected in Jurkat cells, Raji cells, RAW 264.7 cells, RAW264.7 cells. Positive IP detected in Jurkat cells. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, IHC, FC, IP, IF, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
70 kDa zeta assOCiated protein antibody; FLJ17670 antibody; FLJ17679 antibody; SRK antibody; STD antibody; Syk related tyrosine kinase antibody; Tyrosine protein kinase ZAP 70 antibody; TZK antibody; ZAP 70 antibody; ZAP70 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ZAP70 recombinant protein (Accession Number: BC039039). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116905(ZAP70 Antibody) at dilution of 1:3000
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:116905(ZAP70 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:116905(ZAP70 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-ZAP70 (IP:Catalog No:116905, 4ug; Detection:Catalog No:116905 1:1000) with Jurkat cells lysate 2800ug.
Immunofluorescent analysis of RAW 264.7 cells using Catalog No:116905(ZAP70 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 Raji cells were stained with 0.2ug ZAP70 antibody (Catalog No:116905, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
ZAP70 represents zeta-chain (TCR) associated protein kinase 70kDa
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"