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Product Name
Anti-ZAP70 (7G11) Mouse antibody
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Description
ZAP70 (7G11) Mouse monoclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human ZAP70 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ELISA: 1/10000
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Validations
Immunohistochemical analysis of paraffin-embedded human lymph tumor, showing cytoplasmic localization using ZAP70 mouse mAb with DAB staining
Western blot detection of ZAP-70 in Jurkat cell lysates using ZAP-70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
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Background
Swiss-Prot Acc.P43403.ZAP70 (zeta-chain associated protein kinase), a 70 kDa member of the SYK tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. ZAP70 controls TCR(T-cell antigen receptor)-linked signal transduction pathways. Its key role in thymocytes development and mature T lymphocytes activation has been illustrated by the characterization of several human immunodeficiencies presenting with mutations in the ZAP70 gene. ZAP70 is also expressed in several types of B-cell neoplasm and is easily detected by immunohistochemistry, providing a useful prognostic marker in patients with chronic lymphocytic leukaemia.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"