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Product Name
ZAP70 antibody
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Description
ZAP70 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, A375 cells. Positive IP detected in Jurkat cells. Positive IHC detected in human tonsillitis tissue, human kidney tissue, human lymphoma tissue, human testis tissue. Observed molecular weight by Western-blot: 70kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
70 kDa zeta assOCiated protein antibody; FLJ17679 antibody; SRK antibody; STD antibody; Syk related tyrosine kinase antibody; Tyrosine protein kinase ZAP 70 antibody; TZK antibody; ZAP 70 antibody; ZAP70 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ZAP70 recombinant protein (Accession Number: BC039039). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116904(ZAP70 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human tonsillitis using Catalog No:116904(ZAP70 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human tonsillitis using Catalog No:116904(ZAP70 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-ZAP70 (IP:Catalog No:116904, 4ug; Detection:Catalog No:116904 1:1000) with Jurkat cells lysate 3200ug.
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Background
Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.
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